Autolysin (lytA) recombinant protein: a potential target for …
Materials and Methods. DNA was extracted from S. pneumoniae ATCC 49619 to amplify lytA gene by polymerase chain reaction assay.The lytA amplicon and pET28a vector were separately double digested using Nde-1 and Xho1 restriction enzymes and then ligated together with ligase enzyme.The recombinant plasmid was expressed in …